ABSTRACTReceived Jun 23, 2023Revised Aug15, 2023Accepted Sep 8, 2023Published Apr1, 2024Objective: This study aimed to investigate the impact of administering jackfruit seeds milk on hemoglobin levels in third trimester pregnant women at BangetayuHealth Center in Semarang, Indonesia.Materials and Methods: This study utilized a quasi-experimental approach with pre-and post-test control groups. The study included a sample of 35 out of 107 third trimester pregnant women from the Bangetayu HealthCenter in Semarang, Indonesia. The study was conducted between January and February 2022. The research sample comprised 35 respondents, divided into 17 control groups and 18 intervention groups. Purposive sampling was utilized as the samplingtechnique. Dataanalysis involved both univariate and bivariate analysis techniques. The statistical analyses used werechi-square test and independent t-test. This study utilized the SPSS software version 23 for data analysis.Results: The intervention group had an average hemoglobin level of 10.2 g/dL before treatment and 10.7 g/dL after treatment. The average hemoglobin level in the control group increased from 12.1g/dL before to 12.4 g/dL after. The results revealed p=0.000 and a Relative Risk (RR) of 1.2.Conclusion: The consumption of jackfruit seed milk increasedhemoglobin levels of third-trimester pregnant women at the health center in Bangetayu, Semarang, Indonesia. Failure to consume jackfruit seed milk resultedin 1.2 timesdecrease in the likelihood of boosting hemoglobin levels.